Sanger DNA Sequencing, From Then to Now.
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2023-05-30に共有
We'll also compare Sanger sequencing to other sequencing technologies, such as NGS, to give you a better understanding of how it all works. Throughout the video, we'll take a chronological approach to explore the history of Sanger sequencing, starting with its development in the late 1970s and following its evolution. By the end of the video, you'll deeply understand Sanger sequencing and its role in sequencing the human genome.
So grab a cup of coffee and journey through the fascinating world of Sanger sequencing! Whether you're a student, researcher, or just curious about the science behind DNA sequencing, this video is for you!
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📖 This video is also a blog post with images, references and a PDF download; visit: www.clevalab.com/post/sanger-dna-sequencing
👉 You may also like: "Next Generation Sequencing - A Step-By-Step Guide to DNA Sequencing." • Next Generation Sequencing - A Step-B...
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CHAPTERS:
00:00 The 1977 Invention of Sanger Sequencing
00:23 The Basics of How DNA Is Copied
00:36 What are dNTPs & ddNTPs anyway?
01:19 How Does a ddNTP Stop DNA Copying?
01:54 The DNA Naming Convention 5' to 3'
02:43 Which Bases Go Together in Base Pairing?
03:05 Steps of the First Sanger Sequencing Method
06:11 The First DNA Sequencing Instrument, the AB370A.
07:20 The Launch of the Human Genome Project
07:46 How Does Cycle Sequencing Improve Things?
08:59 Automated DNA Separation by Capillary Electrophoresis
09:36 ABI PRISM 310, Modern Sanger Sequencing
10:49 Fluorescent ddNTPs and the Invention of BigDyes
11:33 The Instrument That Sequenced the Genome
13:09 Why Use Sanger Sequencing When There's NGS?
#ClevaLab #sequencing #sangersequencing
コメント (21)
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Welcome to ClevaLab - if you like the video, please give it a 👍and subscribe for more videos. Also, if you have any questions, feel free to ask in the comments. 🤓
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Thank you so much, you can't imagine how much you have helped me. I have been confused for more an year and ths cleared everything
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Loved it.. Perfect description of old methods to the new ones. Videos like this are much needed to understand these concepts visually.
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The animations used in this video are very helpful and well done. Excellent overview of Sanger sequencing.
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Compared to so many materials, this one really made me understand it . Thanks a lot.
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❤this is so good, composed & clear... Thanks
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Very deatiled and informative video with excellent visuals, thank you!!!
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Your Videos are so helpful 😊.Thanks a lot ❤
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This is brilliant. Thank you so much
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thabk you so much! This is so clear!
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This is one of the best videos I have seen in biotech so far.
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Wow! You explained so well. It's easy to understand. You should be a good teacher. Thank you
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Amazing video :D thank you very much 💛
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Great video!
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Can't thank you enough!!!❤
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Best and detailed video
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wow, just a fantastic video thank you.🥰
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I studied Microbiology at university from '93-'98 and this was all a black box. I appreciate the depth of this exploitation. Thank you!
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Hey.. your videos are great. would love to see more of your videos. I am not finding many here. Please upload more videos. May be on fourth-generation technologies ;)
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Amazing video, I got to know about many things yhrough this. I am currently trying to get to know about how to write research paper and for that I chose a topic of next generation sequencing can you provide me a pdf for that purpose?
